Effect of IL-15 on the proliferation, differentiation and anti-apoptosis of CD34+ cells in patients with MDS / 中国实验血液学杂志
Journal of Experimental Hematology
;
(6): 620-623, 2005.
Artigo
em Chinês
| WPRIM
| ID: wpr-356502
ABSTRACT
To study the effect of interleukin-15 (IL-15) on the proliferation, differentiation and apoptosis of MDS CD34(+) cells, CD34(+) cells of high enrichment were separated by MACS system, and cultured in liquid media with different concentration of IL-15 in treated group and without IL-15 in the control group. Apoptosis of hematopoietic precursors was assayed by propidium iodine staining and cell by FCM, and the other MDS CD34(+) cells were stained by cytochemical staining after culture. The results showed that after culture with IL-15 the proliferation and differentiation of MDS CD34(+) cells were obviously promoted. It was found the every lineage of mature cells developed, the expressions of cell surface antigens CD71, CD33 and CD19 all increased in the MDS CD34(+) cell treated with IL-15. It is suggested that IL-15 stimulates the proliferation and differentiation of MDS CD34(+) cells, and partly shows anti-apoptosis effects which may be applicable to the therapy MDS.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Patologia
/
Farmacologia
/
Sangue
/
Síndromes Mielodisplásicas
/
Receptores da Transferrina
/
Células da Medula Óssea
/
Antígenos de Diferenciação Mielomonocítica
/
Antígenos CD
/
Ciclo Celular
/
Diferenciação Celular
Limite:
Humanos
Idioma:
Chinês
Revista:
Journal of Experimental Hematology
Ano de publicação:
2005
Tipo de documento:
Artigo
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