Sequence analysis of 3'-non-coding region of RHD / 中国实验血液学杂志
Journal of Experimental Hematology
;
(6): 219-221, 2005.
Artigo
em Chinês
| WPRIM
| ID: wpr-356589
ABSTRACT
There are 3' non-coding region, downstream Rhesus box, SMP1 gene et cetera. after RHD stop code. This study was intended to determine the sequence of 3' non-coding region. One pairs of primer was designed and then a polymerase chain reaction (PCR) was established for specific amplification of whole length of 3' non-coding region of RHD in 10 Rh-positive and 10 D(el) samples. The PCR products were purified and directly sequenced. The results showed that all Rh-positive and D(el) samples were identical, which revealed that there were 103 bp between 3'-end of RHD coding region and 5'-end of downstream Rhesus box. The D(el) samples showed the same result with the normal Rh-positive sample. It suggests that lower expression of D antigen in D(el) red cells does not associate with 3' non-coding region of D(el) gene.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Sistema do Grupo Sanguíneo Rh-Hr
/
Dados de Sequência Molecular
/
Sequência de Bases
/
Análise de Sequência de DNA
/
Regiões 3' não Traduzidas
/
Genética
Limite:
Humanos
Idioma:
Chinês
Revista:
Journal of Experimental Hematology
Ano de publicação:
2005
Tipo de documento:
Artigo
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