Expansion in vitro and cytotoxicity of dendritic cells from patients with chronic myeloid leukemia / 中国实验血液学杂志
Journal of Experimental Hematology
; (6): 198-204, 2005.
Article
em En
| WPRIM
| ID: wpr-356593
Biblioteca responsável:
WPRO
ABSTRACT
The study was aimed to investigate the extensive amplification and the cytotoxicity of dendritic cells (DC) derived from chronic myeloid leukemia cells. DC were cultured in two steps: firstly, extensive amplification in primary culture of CD34(+) or mononuclear cells isolated from CML patients' bone marrow and peripheral blood with rhFlt3-L and rhTPO for 7 days; secondly, inducing culture of DC with rhGM-CSF, rhTNF and rhIL-4 for 14 days. A system inducing DC directly were established for comparison. DC were identified by immunophenotype with flow cytometry, chromosome analysis by displaying G banding and electric microscopy analysis. The function of stimulating T cells proliferation and cytotoxicity of CML cells were confirmed through MTT assay. The results showed that after first extensive amplification in primary culture with rhFlt3-L and rhTPO for 7 days, CD34(+) cells had a total cell number with (77 +/- 5) fold expansion, and DC were (39 +/- 8)% of total cell respectively after induction culture of DC with rhGM-CSF, rhTNF and rhIL-4 for 14 days. Both the amplification of cell number and yield of DC were higher than the system without extensively culture (P < 0.01). Such DC could stimulate T cells to proliferate and kill leukemia cells finally. In conclusion, two-step culture method can obviously improve the cell number of DC required, that is better than inducing them directly. DC derived from CML cells induce the generation of anti-leukemia immunization.
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Índice:
WPRIM
Assunto principal:
Patologia
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Células Dendríticas
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Linfócitos T Citotóxicos
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Leucemia Mielogênica Crônica BCR-ABL Positiva
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Células Cultivadas
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Imunofenotipagem
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Antígenos CD34
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Citotoxicidade Imunológica
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Proliferação de Células
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Alergia e Imunologia
Limite:
Adolescent
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Adult
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Aged
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Child
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Female
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Humans
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Male
Idioma:
En
Revista:
Journal of Experimental Hematology
Ano de publicação:
2005
Tipo de documento:
Article