Genome amplification and sequence analysis of human bocavirus 2 / 病毒学报
Chinese Journal of Virology
;
(6): 257-262, 2014.
Artigo
em Chinês
| WPRIM
| ID: wpr-356606
ABSTRACT
To obtain the genome sequence of human bocavirus 2 (HBoV2), different regions of HBoV2 genome were amplified through PCR in fecal specimens which had been identified as single-positive for HBoV2 in 2010. A genome sequence of HBoV2 (HBoV2-NC, 5444 bp) was obtained after sequence assembly. The phylogenetic analysis showed that HBoV2-NC had the closest evolutionary relationship with HBoV2 Lanzhou strain. The predication of inverted terminal repeats of HBoV2-NC by DINAMelt showed that inverted terminal repeats were contained in HBoV2-NC 5' terminal, which had the typical stem-loop structure in other parvoviruses. Finally, some flanking sequences of HBoV2-NC were amplified by linker-PCR.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Filogenia
/
Virologia
/
RNA Viral
/
Dados de Sequência Molecular
/
Sequência de Bases
/
Química
/
Amplificação de Genes
/
Classificação
/
Genoma Viral
/
Infecções por Parvoviridae
Limite:
Humanos
Idioma:
Chinês
Revista:
Chinese Journal of Virology
Ano de publicação:
2014
Tipo de documento:
Artigo
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