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Morphogenetic study of human adenovirus type 41 in 293TE cells / 病毒学报
Chinese Journal of Virology ; (6): 154-161, 2014.
Artigo em Chinês | WPRIM | ID: wpr-356622
ABSTRACT
To investigate the morphogenetic process of human adenovirus type 41 (HAdV-41), 293TE cells were infected with purified wild-type HAdV-41, and ultrathin sections of infected cells were prepared and observed under a transmission electron microscope. Results showed that HAdV-41 entered host cells mainly through three ways non-clathrin-coated pit, clathrin-coated pit, and direct penetration of plasma membrane. In addition, cell microvilli might help HAdV-41 enter cells. After entering into cells, HAdV-41 virus particles could be found in vacuoles or lysosomes or be in a free state in cytoplasm. Only free virus particles could be found near nuclear pores (NP), suggesting that the virus needed to escape from lysosomes for effective infection and viral nucleoprotein entered the nucleus through NP. Progeny viruses were as-sembled in the nucleus. Three types of inclusion bodies, which were termed as fibrillous inclusion body, condense inclusion body, and stripped condense inclusion body, were involved in HAdV-41 morphogenesis. In the late phase of viral replication, the membrane integrity of the infected cells was lost and viral particles were released extracellularly. This study reveals the partial process of HAdV-41 morphogenesis and provides more biological information on HAdV-41.
Assuntos
Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Fisiologia / Virologia / Replicação Viral / Membrana Celular / Núcleo Celular / Infecções por Adenovirus Humanos / Adenovírus Humanos / Liberação de Vírus / Genética Limite: Humanos Idioma: Chinês Revista: Chinese Journal of Virology Ano de publicação: 2014 Tipo de documento: Artigo

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Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Fisiologia / Virologia / Replicação Viral / Membrana Celular / Núcleo Celular / Infecções por Adenovirus Humanos / Adenovírus Humanos / Liberação de Vírus / Genética Limite: Humanos Idioma: Chinês Revista: Chinese Journal of Virology Ano de publicação: 2014 Tipo de documento: Artigo