The novel multiplex PCRs and their applications in respiratory viruses diagnosis / 病毒学报
Chinese Journal of Virology
;
(6): 638-645, 2013.
Artigo
em Chinês
| WPRIM
| ID: wpr-356654
ABSTRACT
Acute respiratory tract infections (ARTIs) are widely distributed among the population, mainly caused by respiratory viruses. ARTIs are responsible for significant morbidity and mortality among the elderly and infants or young children, causing a serious economic burden. The rapid and accurate identifi cation of a pathogen will provide a guideline for the clinical diagnosis and therapy. Multiplex polymerase chain reaction (PCR) technologies combine the rapidness and high sensitivity of PCR with high through put, thus achieving the capability of detecting multiple pathogens simultaneously. The commercial kits based on these multiplex PCR methods allow to detect more than twelve respiratory viruses simultaneous ly, reaching the comparable sensitivities and specificities to those of real-time PCR. The recent progress of novel multiplex PCR assays and their principles as well as applications in respiratory virus diagnosis were reviewed in this paper.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Infecções Respiratórias
/
Virologia
/
Vírus
/
Viroses
/
Classificação
/
Diagnóstico
/
Reação em Cadeia da Polimerase Multiplex
/
Genética
/
Métodos
Tipo de estudo:
Estudo diagnóstico
/
Guia de Prática Clínica
Limite:
Animais
/
Humanos
Idioma:
Chinês
Revista:
Chinese Journal of Virology
Ano de publicação:
2013
Tipo de documento:
Artigo
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