Determination of cyclovirobuxine-D in huangyangning tablets by RP-HPLC with gradient elution / 中国中药杂志

ABSTRACT

<p><b>OBJECTIVE</b>To establish a new method for the determination of cyclovirobuxine-D in huangyangning tablets.</p><p><b>METHOD</b>Agilent Zorbax Extend C18 (4.6 mm x 250 mm, 5 microm) column with temperature 40 degrees C was used. The mobile phase was 0.3% diethylamine methanol solution and 0.3% diethylamine water solution, with a linear gradient of 0.3% diethylamine methanol solution from 78% to 95% in 9 minutes, and then maintained for 6 minutes. The flow rate was 0.8 mL x min(-1). Evaporative light scattering detector was used.</p><p><b>RESULT</b>The calibration curve was linear at a range of 0.57-11.44 microg for the cyclovirobuxine D (r = 0.9996). The average recovery was 97.2% and RSD was 1.1% (n = 9).</p><p><b>CONCLUSION</b>This method is rapid, simple, and reproducible, and can be used as a quality control method for this preparation.</p>