Effect of vitexia-rhamnoside (V-R) on vasomotor factors expression of endothelial cell / 中国中药杂志
China Journal of Chinese Materia Medica
; (24): 566-569, 2006.
Article
em Zh
| WPRIM
| ID: wpr-356767
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To observe the influence of vitexia-rhamnoside (V-R) on vasomotor factor expression of endothelial cell (EC) damaged by hypoxia and reoxygenation.</p><p><b>METHOD</b>The cultured human umbilical vein endothelial cells (HUV ECs) were subject to ischemia and reperfusion following hypoxia and reoxygenation. The levels of ET-1, NO and NOS intracellular in culture supertanants were measured by radioimmunity, Griess and immunohistochemistry, respectively. And the gene expressions of ET-1 and NOS intracellular were measured by reverse transcriptase-polymerase chain reaction.</p><p><b>RESULT</b>V-R at different doses markedly increased the gene expression and activity of NOS, enhanced the level of vaso-dilating factor NO, and significantly decreased the gene expression and production of vaso-constricting factor ET-1 of EC.</p><p><b>CONCLUSION</b>We have demonstrated that V-R had the regulatory effect on the expression of vaso-active substances of EC damaged by hypoxia and reoxygenation in the levels of protein and gene transcription of cytokines.</p>
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Índice:
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Assunto principal:
Farmacologia
/
Plantas Medicinais
/
Veias Umbilicais
/
RNA Mensageiro
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Hipóxia Celular
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Expressão Gênica
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Células Cultivadas
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Química
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Folhas de Planta
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Óxido Nítrico Sintase
Limite:
Humans
Idioma:
Zh
Revista:
China Journal of Chinese Materia Medica
Ano de publicação:
2006
Tipo de documento:
Article