Effects of red orpiment on cell morphology and expression of PML mRNA and protein in NB4 and HL-60 cells / 中华医学杂志(英文版)
Chinese Medical Journal
;
(24): 148-150, 2003.
Artigo
em Inglês
| WPRIM
| ID: wpr-356848
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effects of red orpiment on cell morphology, expression of promyelocytic leukemia (PML) mRNA and its protein localization in NB4 and HL-60 cell lines.</p><p><b>METHODS</b>Cell morphology was assayed by Wright's staining and fluorescence staining, while PML mRNA expression was determined by RT-PCR. PML protein localization by evaluated by immunofluorescence staining.</p><p><b>RESULTS</b>The typical apoptosis was found in NB4 and HL-60 cells after treatment with red orpiment. The fusion protein was no longer observed in NB4 cells, PML protein was relocated, and then degraded. In HL-60 cells, PML protein underwent a similar progress. The expression of promyelocytic leukemia (PML) mRNA was not changed in the treated cells.</p><p><b>CONCLUSION</b>Red orpiment inhibits the proliferation of leukemia cells by inducing them to undergo apoptosis.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Patologia
/
Farmacologia
/
Arsenicais
/
Fatores de Transcrição
/
RNA Mensageiro
/
Proteínas Nucleares
/
Células Tumorais Cultivadas
/
Leucemia Promielocítica Aguda
/
Apoptose
/
Células HL-60
Tipo de estudo:
Estudo diagnóstico
Limite:
Humanos
Idioma:
Inglês
Revista:
Chinese Medical Journal
Ano de publicação:
2003
Tipo de documento:
Artigo
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