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Polysaccharide sulfate 916 inhibits neutrophil-endothelial adhesion / 中华医学杂志(英文版)
Chinese Medical Journal ; (24): 1855-1858, 2002.
Artigo em Inglês | WPRIM | ID: wpr-356893
ABSTRACT
<p><b>OBJECTIVE</b>To study the effect of polysaccharide sulfate 916 (PS916) on neutrophil-endothelial cell adhesion.</p><p><b>METHODS</b>Cell adhesion was evaluated by testing neutrophil myeloperoxidase activity. Expression of adhesion molecule in human umbilical vein endothelial cell (HUVEC) was measured by ELISA. The neutrophil activation rate induced by N-formyl-methionyl-leucyl-phenylalanine (fMLP) was tested by nitroblue tetrazolium (NBT) reduction.</p><p><b>RESULTS</b>Tumor necrosis factor alpha (TNFalpha, 50 - 800 U/ml) increased the adherence of neutrophil to TNFalpha-stimulated HUVEC in a concentration and time dependent manner. PS916 (0.01 - 1.0 mg/ml) dose-dependently inhibited the adherence of neutrophils to TNFalpha-stimulated HUVEC. fMLP increased the activation rate of neutrophils independent of concentration. PS916 also inhibited the adherence of fMLP-activated neutrophils to HUVEC. Moreover, PS916 inhibited adhesion molecule expression in TNFalpha-stimulated HUVEC.</p><p><b>CONCLUSIONS</b>PS916 inhibited neutrophil-endothelial adhesion. The mechanism of its action was partially related to suppressing the expressions of intercellular adhesion molecule-1 (ICAM-1) and vascular adhesion molecule-1 (VCAM-1).</p>
Assuntos
Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Farmacologia / Fisiologia / Polissacarídeos / Ácidos Sulfúricos / Endotélio Vascular / Adesão Celular / Células Cultivadas / Fator de Necrose Tumoral alfa / Ratos Wistar / Molécula 1 de Adesão Intercelular Limite: Animais / Humanos Idioma: Inglês Revista: Chinese Medical Journal Ano de publicação: 2002 Tipo de documento: Artigo

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Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Farmacologia / Fisiologia / Polissacarídeos / Ácidos Sulfúricos / Endotélio Vascular / Adesão Celular / Células Cultivadas / Fator de Necrose Tumoral alfa / Ratos Wistar / Molécula 1 de Adesão Intercelular Limite: Animais / Humanos Idioma: Inglês Revista: Chinese Medical Journal Ano de publicação: 2002 Tipo de documento: Artigo