miR-221 mediates epithelial-mesenchymal transition-related gene expressions via regulation of PTEN/Akt signaling in drug-resistant glioma cells / 南方医科大学学报
Journal of Southern Medical University
;
(12): 218-222, 2014.
Artigo
em Chinês
| WPRIM
| ID: wpr-356951
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the correlation between miR-221 and epithelial-mesenchymal transition (EMT) in drug-resistant glioma cells.</p><p><b>METHODS</b>The expression levels of miR-221, PTEN, p-Akt, E-cadherin, vimentin, and MRP1 were quantitatively analyzed in Z1 cells (primary drug-resistant cells), Z2 cells (drug-sensitive cells) and Z2-BCNU cells (drug-resistant cells) using fluorescent real-time PCR and Western blotting.</p><p><b>RESULTS</b>The expression levels of PTEN were significantly increased in Z2 cells compared with Z1 and Z2-BCNU cells which overexpressed miR-221 and vimentin. The expression levels of vimentin, p-Akt and MRP1 were significantly decreased in Z2 cells overexpressing E-cadherin.</p><p><b>CONCLUSION</b>MiR-221 regulates the expression of EMT-related genes through down-regulation of PTEN and activation of PI3-K/Akt signaling.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Vimentina
/
Transdução de Sinais
/
Caderinas
/
Regulação Neoplásica da Expressão Gênica
/
Resistencia a Medicamentos Antineoplásicos
/
Proteínas Associadas à Resistência a Múltiplos Medicamentos
/
MicroRNAs
/
Linhagem Celular Tumoral
/
PTEN Fosfo-Hidrolase
/
Proteínas Proto-Oncogênicas c-akt
Limite:
Humanos
Idioma:
Chinês
Revista:
Journal of Southern Medical University
Ano de publicação:
2014
Tipo de documento:
Artigo
Similares
MEDLINE
...
LILACS
LIS