Effects of pioglitazone on myocardial peroxisome proliferator-activated receptor gamma co-activator lα expression in rats with myocardial ischemia/reperfusion injury / 南方医科大学学报

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the effects of pioglitazone on the expression of peroxisome proliferator-activated receptor gamma co-activator lα (PGC-lα) in rat myocardium following myocardial ischemia/reperfusion (I/R) injury.</p><p><b>METHODS</b>Twenty-four SD rats were randomly divided into 4 equal groups, namely I/R group, pioglitazone (5 mg/kg daily) group, pioglitazone (10 mg/kg daily) group, and pioglitazone (10 mg/kg) +peroxisome proliferator-activated receptor γ (PPARγ)-specific antagonist GW9662 group. Myocardial I/R injury was induced by ligation of the left anterior descending coronary artery for 30 min and reperfusion for 120 min. Myocardial apoptosis following I/R injury was examined with TUNEL assay; RT-PCR and Western blotting were employed to detect the expression of PGC-lα mRNA and protein, respectively.</p><p><b>RESULTS</b>Pioglitazone treatment significantly suppressed myocardial apoptosis (21.4%∓8.8%,17.3%∓8.7%, 40.1%∓12.3%, P<0.05) following I/R injury and up-regulated myocardial PGC-lα expression at both the mRNA and protein levels (P<0.05), but these effects were antagonized by GW9662 (P<0.05).</p><p><b>CONCLUSION</b>Pioglitazone can inhibit myocardial apoptosis induced by I/R injury and up-regulate myocardial PGC-lα expression, and these effects are mediated by PPARγ.</p>