Overexpression of Wnt3 inhibits apoptosis of hepatic progenitor cells in vitro / 南方医科大学学报
Journal of Southern Medical University
;
(12): 46-50, 2014.
Artigo
em Chinês
| WPRIM
| ID: wpr-356989
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effects of adenoviral vector-mediated over-expression of Wnt3 on the apoptosis of hepatic progenitor cells in vitro.</p><p><b>METHODS</b>Hepatic progenitor cells transfected with Ad-GFP-Wnt3 vector or the control vector Ad-GFP were examined for cell apoptosis under fluorescence microscopy with Hoechst 33342 staining, and the proportion of apoptotic cells were determined by flow cytometric analysis with Annexin-PE/7-ADD staining. The mRNA and protein expressions of Bax, Bcl-2 and Bcl-xl in the cells were detected by real-time PCR and Western blotting, respectively.</p><p><b>RESULTS</b>Real-time PCR and Western blotting showed a high expression of Wnt3 in Ad-GFP-Wnt3-transfected hepatic progenitor cells, which exhibited significantly decreased cell apoptosis as compared with the control group. The expressions of Bcl-2 and Bcl-xl mRNA and proteins increased significantly while Bax expression decreased obviously in Ad-GFP-Wnt3-transfected cells (P<0.05).</p><p><b>CONCLUSIONS</b>Adenoviral vector-mediated over-expression of Wnt3 can suppress apoptosis of hepatic progenitor cells possibly through the Bcl-2 pathway.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Células-Tronco
/
Transfecção
/
Células Cultivadas
/
Apoptose
/
Proteínas Proto-Oncogênicas c-bcl-2
/
Hepatócitos
/
Biologia Celular
/
Proteína bcl-X
/
Proteína Wnt3
/
Vetores Genéticos
Limite:
Humanos
Idioma:
Chinês
Revista:
Journal of Southern Medical University
Ano de publicação:
2014
Tipo de documento:
Artigo
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