Cloning expression of a novel human protein-disulfide isomerase like cDNA / 生物医学工程学杂志
Journal of Biomedical Engineering
;
(6): 459-462, 2002.
Artigo
em Chinês
| WPRIM
| ID: wpr-357002
ABSTRACT
Using the large scale sequencing, a novel human cDNA of 1,645 bp was screened from the cDNA library of human fetal brain. The cDNA contains an ORF encoding a 296-aa protein with a calculated molecular weight of 34.0 KD. Compared with that of current sequence databases, the putative protein was found to have 36% homology with protein-disulfide isomerase (PDI). So this cDNA was named PDI like (PDI-L) gene. Multiple tissue Northern blot analysis shows that PDI-L cDNA is expressed in heart, brain, liver, kidneys and so on. The ORF fragment of PDI-L cDNA was inserted into reconstructed pBV220 expression vehicle and its predicted expressive protein was obtained.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Sequência de Bases
/
Biblioteca Gênica
/
Sequência de Aminoácidos
/
Clonagem Molecular
/
DNA Complementar
/
Isomerases de Dissulfetos de Proteínas
/
Genética
Limite:
Humanos
Idioma:
Chinês
Revista:
Journal of Biomedical Engineering
Ano de publicação:
2002
Tipo de documento:
Artigo
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