Fusion expression of Neisseria gonorrhoeae outmembrane protein with a mucosal adjuvant / 中华男科学杂志

ABSTRACT

<p><b>OBJECTIVE</b>To express a fusion protein of Neisseria gonorrhoeae with a mucosal adjuvant.</p><p><b>METHODS</b>The gene coding Loop VI-VIII(PL678) of porin, an out-membrane protein of Neisseria gonorrhoeae, was obtained by PCR. It was inserted into a plasmid fused with subunit B of heat labile enterotoxin. The recombinant was transformed in E. coli. The expression of fusion protein was analysed by ELISA, SDS-PAGE and Western-blot.</p><p><b>RESULT</b>Fusion protein with LTB was successfully expressed, and displayed both the ability of binding GM1 and the reactogenicity with polyclonal antibodies against Neisseria gonorrhoeae.</p><p><b>CONCLUSION</b>The expression of fusion protein laid a foundation for the study of the intramolecular vaccine against Neisseria gonorrhoeae.</p>