Effect of microRNA143 expression on cell proliferation in colonic carcinoma / 中华肿瘤杂志
Chinese Journal of Oncology
;
(12): 498-501, 2008.
Artigo
em Chinês
| WPRIM
| ID: wpr-357389
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of microRNA143 on cell proliferation and K-ras expression in colorectal carcinoma.</p><p><b>METHODS</b>Northern blot was used to examine the expression of miR-143 in colorectal carcinoma and adjacent normal tissues. A miR-143 expression vector was constructed and transfected into a human colon adenocarcinoma cell line SW480. Cell proliferation was evaluated by MTT assay. RT-PCR and Western blot were used to examine the expression of K-ras oncogene in transfected cells.</p><p><b>RESULTS</b>The level of mature miR-143 was lower in tumors compared with adjacent normal tissues in 81% of colorectal carcinoma specimens. In transfected cells, the increased accumulation of miR-143 inhibited the cell proliferation, and resulted in approximately 40.3% decrease of K-ras protein levels, but had no effect on level of K-ras mRNA.</p><p><b>CONCLUSION</b>The increased accumulation of miR-143 inhibits the proliferation of transfected cells, and results in down-regulation of K-ras protein in colorectal carcinoma.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Patologia
/
Plasmídeos
/
RNA Mensageiro
/
Transfecção
/
Adenocarcinoma
/
Regulação para Baixo
/
Genes ras
/
Neoplasias do Colo
/
Proteínas ras
/
MicroRNAs
Limite:
Humanos
Idioma:
Chinês
Revista:
Chinese Journal of Oncology
Ano de publicação:
2008
Tipo de documento:
Artigo
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