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Effects of cytosolic bacteria on cyclic GMP-AMP synthase expression in human gingival tissues and periodontal ligament cells / 华西口腔医学杂志
West China Journal of Stomatology ; (6): 203-207, 2017.
Artigo em Chinês | WPRIM | ID: wpr-357494
ABSTRACT
<p><b>OBJECTIVE</b>This work aims to determine the effect of cytosolic bacteria on the expression of cyclic GMP-AMP synthase (cGAS) in human periodontal ligament cells (hPDLCs) and gingival tissues.</p><p><b>METHODS</b>The ability of Porphyromonas gingivalis (P. gingivalis) to invade hPDLCs was detected using laser scanning confocal microscope assay at a multiplicity of infection of 10. P. gingivalis-infected cells were sorted by fluorescence-activated cell sorting (FACS). Then, quantitative real time reverse transcription polymerase chain reaction (qRT-PCR) and Western blot were used to detect cGAS expression in infected cells. Finally, the location and expression of cGAS in inflammatory and normal gingival tissues were investigated by immunohistochemistry.</p><p><b>RESULTS</b>P. gingivalis actively invaded hPDLCs. Moreover, cGAS expression significantly increased in P. gingivalis-infected cells. Although cGAS was expressed in the epithelial and subepithelial cells of both inflamed and normal gingival tissues, cGAS expression significantly increased in inflamed gingival tissues.</p><p><b>CONCLUSIONS</b>Cytosolic bacteria can upregulate cGAS expression in infected cells. These data suggest that cGAS may act as pattern-recognition receptors and participate in recognizing cytosolic nucleic acid pathogen-associated molecular patterns.
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Assuntos

Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Ligamento Periodontal / Células Cultivadas / Western Blotting / Porphyromonas gingivalis / Reação em Cadeia da Polimerase em Tempo Real / Citometria de Fluxo / Gengiva / Nucleotídeos Cíclicos Limite: Humanos Idioma: Chinês Revista: West China Journal of Stomatology Ano de publicação: 2017 Tipo de documento: Artigo

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Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Ligamento Periodontal / Células Cultivadas / Western Blotting / Porphyromonas gingivalis / Reação em Cadeia da Polimerase em Tempo Real / Citometria de Fluxo / Gengiva / Nucleotídeos Cíclicos Limite: Humanos Idioma: Chinês Revista: West China Journal of Stomatology Ano de publicação: 2017 Tipo de documento: Artigo