Cadherin switching induced by P120-catenin can promote the migration and invasion of oral squamous cell cancer cells / 华西口腔医学杂志
West China Journal of Stomatology
;
(6): 183-186, 2017.
Artigo
em Chinês
| WPRIM
| ID: wpr-357498
ABSTRACT
<p><b>OBJECTIVE</b>The main goal is to investigate the role of P120-catenin (P120ctn) in cadherin switching, as well as migration and invasion, of oral squamous cell cancer (OSCC) cells.</p><p><b>METHODS</b>The plasmid pGFP-V-RS-P120ctn shRNA was used to transfect TSCCA cells and significantly reduce the expression of P120ctn in these cells. Real-time fluorescent quantitative polymerase chain reaction and Western blot were conducted to determine the mRNA and protein expression levels of P120ctn, E-cadherin (E-cad), and N-cadherin (N-cad). By contrast, the Transwell cell invasion and cell migration assay was used to determine the invasion and migration capacities before and after the transfection.</p><p><b>RESULTS</b>After the plasmid pGFP-V-RS-P120ctn shRNA was transfected into the TSCCA cells, we found that as the P120ctn expression significantly decreased, E-cad mRNA and protein expression decreased significantly. Moreover, N-cad mRNA and protein expression increased significantly (P<0.05). Lastly, the cell migration and invasion capacities were augmented significantly (P<0.05).</p><p><b>CONCLUSIONS</b>In OSCC cells, P120ctn may be involved in cadherin switching and promote metastasis and invasion.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Neoplasias Bucais
/
Carcinoma de Células Escamosas
/
Transfecção
/
Caderinas
/
Movimento Celular
/
Linhagem Celular Tumoral
/
Cateninas
/
Invasividade Neoplásica
/
Metástase Neoplásica
Limite:
Humanos
Idioma:
Chinês
Revista:
West China Journal of Stomatology
Ano de publicação:
2017
Tipo de documento:
Artigo
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