The tests of acute toxicity

ABSTRACT

There are some inapropriate problems 1. The number of mice according to method from 30-100; 2. The weight of mice is 16-22g but most common is 19+/-1g(45 days);3. If oral dosage 0.2 ml/10 g mice, if body weight19+/- 1 g the same dose of 0.4-0.5 ml/mice; 4. As calculating method of Behrens, the number of mice can provide LD 50 with small adds; 5. Safe maximal dose; 6. The mice is monitored mainly during first 24 hours. The monitoring time may be 36-48 hours; 7. The morphological, frequency and amplitude change of all waves in electrocardiogram and encephalogram should be nomitored.