Identification of antiviral activity of Toddalia asiatica against influenza type A virus / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 998-1001, 2005.
Artigo
em Chinês
| WPRIM
| ID: wpr-358040
ABSTRACT
<p><b>OBJECTIVE</b>To identify antiviral activity of Toddalia asiatica against influenza virus type A in vitro.</p><p><b>METHOD</b>More than two hundred Chinese medicinal herb extracts were screened for antiviral activities against influenza A/PR/8/34 (H1N1) virus using 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) assay for virus induced cytopathic effect (CPE) in a primary screening. Positive samples were picked up and were subjected to quantitative real-time polymerase chain reaction (PCR) to quantify reduction of H1N1 virus genomic RNA.</p><p><b>RESULT</b>Toddalia asiatica showed potent antiviral activities against H1N1 virus, with 50% effective concentration (EC50) value of 4.7 mg x L(-1) in MTS assay and 0.9 mg x L(-1) in quantitative PCR assay respectively. The cytotoxicity test of Toddalia asiatica generated a CC50 value of 187.2 mg x L(-1) and a selective index (SI) larger than 206 in quantitative PCR. Although the best antiviral activity of Toddalia asiatica was observed with co-treatment of influenza virus infection, it remained effective even when administrated 24 h before and after the initiation of infection.</p><p><b>CONCLUSION</b>The results suggested that Toddalia asiatica compound extract could be a candidate for anti-H1N1 virus agent in the treatment of influenza.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Antivirais
/
Farmacologia
/
Plantas Medicinais
/
Fatores de Tempo
/
RNA Viral
/
Medicamentos de Ervas Chinesas
/
Células Cultivadas
/
Química
/
Rutaceae
/
Biologia Celular
Tipo de estudo:
Estudo diagnóstico
/
Estudo prognóstico
Limite:
Animais
Idioma:
Chinês
Revista:
China Journal of Chinese Materia Medica
Ano de publicação:
2005
Tipo de documento:
Artigo
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