Analysis of volatile components from the flowers of Chrysanthemum morifolium by GC-MS with solid-phase microextraction / 中国中药杂志

ABSTRACT

<p><b>OBJECTIVE</b>To study a method for extraction and analysis of volatile components from Chrysanthemum morifolium 'gonghuangjv' cv. nov (CM GHJ) and C. morifolium 'gongbaijv' cv. nov (CM GBJ) by solid-phase microextraction (SPME) and gas chromatography-mass spectrometry (GC-MS).</p><p><b>METHOD</b>The volatile components were extracted in different temperature, different balance period and different extraction fiber using head space solid-phase microextraction (HS-SPME), and were identified by CGC-MS. The variety in integral area of each component was observed in different conditions and its relative content was determined by normalization of area.</p><p><b>RESULT</b>The better condition of SPME for C. morifolium was that the sample was extracted using 100 microm polydimethylsiloxane (PDMS) extraction fiber after it had been balanced for 6 hours at 75 degrees C. 53 components from CM GHJ and CM GBJ were identified, and there were 35 same components in CM GHJ and CM GBJ.</p><p><b>CONCLUSION</b>HS-SPME-GC-MS is convenient, rapid and reliable for analysis of volatile components in C. morifolium.</p>