Effects of mineral cations on the accumulation of tanshinone II A and protocatechuic aldehyde in the adventitious root culture of Salvia niltiorrhiza / 中国中药杂志

ABSTRACT

<p><b>OBJECTIVE</b>To study the effects of mineral cations on the growth of Salvia niltiorrhiza adventitious roots and the accumulation of tanshinone II A and protocatechuic aldehyde.</p><p><b>METHOD</b>The adventitious roots were cultured under different concentrations of metal cations and the contents of tanshinone II A and protocatechuic aldehyde were determined by HPLC.</p><p><b>RESULT</b>Fe2 + and Mn2+ in MS medium are suitable for adventitious root growth, but Cu2+ and Mg2+ in high concentration could improve root proliferation, and Zn2+ has no obvious effect on root growth. As for tanshinone II A and protocatechuic aldehyde biosynthesis, Cu2+ and Zn2+ would inhibit the biosynthesis of protocatechuic aldehyde, furthermore, proper Fe2+ and Mg2+ concentration could advance the biosynthesis of protocatechuic aldehyde, whereas, Mn2+ in high concentration could accelerate the biosynthesis of protocatechuic aldehyde. Cu2+, Zn2+, Mg2+ in low concentration and Fe2+ and Mn2+ in high concentration would all advance the biosynthesis of tanshinone II A.</p><p><b>CONCLUSION</b>The mineral cations have obvious effects on the secondary metabolites biosynthesis in adventitious root culture of S. niltiorrhiza.</p>