Characterization of oral bacterial diversity of irradiated patients by high-throughput sequencing / 国际口腔科学杂志·英文版
International Journal of Oral Science
;
(4): 21-25, 2013.
Artigo
em Inglês
| WPRIM
| ID: wpr-358197
ABSTRACT
The objective of this study was to investigate the compositional profiles and microbial shifts of oral microbiota during head-and-neck radiotherapy. Bioinformatic analysis based on 16S rRNA gene pyrosequencing was performed to assess the diversity and variation of oral microbiota of irradiated patients. Eight patients with head and neck cancers were involved in this study. For each patient, supragingival plaque samples were collected at seven time points before and during radiotherapy. A total of 147,232 qualified sequences were obtained through pyrosequencing and bioinformatic analysis, representing 3,460 species level operational taxonomic units (OTUs) and 140 genus level taxa. Temporal variations were observed across different time points and supported by cluster analysis based on weighted UniFrac metrics. Moreover, the low evenness of oral microbial communities in relative abundance was revealed by Lorenz curves. This study contributed to a better understanding of the detailed characterization of oral bacterial diversity of irradiated patients.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Propionibacteriaceae
/
Efeitos da Radiação
/
Radioterapia
/
Streptococcus
/
Bactérias
/
Veillonella
/
Actinomyces
/
Actinomycetaceae
/
RNA Bacteriano
/
Capnocytophaga
Tipo de estudo:
Estudo observacional
/
Estudo prognóstico
Limite:
Humanos
Idioma:
Inglês
Revista:
International Journal of Oral Science
Ano de publicação:
2013
Tipo de documento:
Artigo
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