Detection of epidermal growth factor receptor gene mutations and its clinical significance in non-small cell lung cancers / 中华病理学杂志
Chinese Journal of Pathology
;
(12): 671-674, 2011.
Artigo
em Chinês
| WPRIM
| ID: wpr-358267
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the detection technology and its clinical significance of the EGFR gene mutation in non-small cell lung cancer.</p><p><b>METHODS</b>DNA direct sequencing methods by PCR amplification were used to detect EGFR gene exons 18-21 mutation and to analyze its clinical pathological significance in 192 patients with non-small cell lung cancer.</p><p><b>RESULTS</b>64 of the 192 cases presented with EGFR gene tyrosine kinase binding domain mutation (64/192, 33.3%), of which exon 19 deletion mutation rate was 60.9% (39/64), exon 21 alternative mutation rate was 39.1% (25/64), but exons 18 and 20 mutation was not found in this group of patients. EGFR gene mutation rate was 58.5%(24/41) in lung adenocarcinoma associated with bronchioloalveolar carcinoma differentiation, which was significantly higher than that of ordinary adenocarcinoma (37.9%, 33/87), squamous cell carcinoma (7.5%, 4/53), large cell carcinoma (1/5) and adenosquamous carcinoma (2/6, P<0.05). EGFR gene mutation rates in male patients (20.9%, 24/115), were significantly higher than in the females (51.9%, 40/77; P<0.01); non-smokers (50.0%, 57/114), significantly higher than that of smokers (9.0%, 7/78; P<0.01).</p><p><b>CONCLUSIONS</b>DNA direct sequencing method by PCR amplification is stable and reliable in detection of EGFR gene mutation in non-small cell lung cancer. It might provide a scientific basis for targeted therapy.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Patologia
/
Adenocarcinoma
/
Fumar
/
Fatores Sexuais
/
Reação em Cadeia da Polimerase
/
Éxons
/
Análise de Sequência de DNA
/
Carcinoma Pulmonar de Células não Pequenas
/
Adenocarcinoma Bronquioloalveolar
/
Genes erbB-1
Tipo de estudo:
Estudo diagnóstico
Limite:
Adulto
/
Idoso
/
Feminino
/
Humanos
/
Masculino
Idioma:
Chinês
Revista:
Chinese Journal of Pathology
Ano de publicação:
2011
Tipo de documento:
Artigo
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