Expression of IgVH and B7-1 in proteome of the human colorectal carcinoma cell lines / 中华肿瘤杂志
Chinese Journal of Oncology
;
(12): 648-652, 2005.
Artigo
em Chinês
| WPRIM
| ID: wpr-358545
ABSTRACT
<p><b>OBJECTIVE</b>To conduct a proteomic analysis of human colorectal carcinoma cell lines LS174T and SW480 by two-dimensional electrophoresis (2-DE) and matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF-MS).</p><p><b>METHODS</b>The total proteins of human colorectal carcinoma cell lines LS174T and SW480 were separated with 2-DE using immobilized pH gradient strips and analyzed by MALDI-TOF-MS to obtain peptide mass fingerprints (PMFs). Proteins were identified by using Mascot software to search protein databases.</p><p><b>RESULTS</b>Good resolution 2-DE maps were obtained. Some proteins including immunoglobulin heavy chain variable region (IgVH) and co-stimulatory molecule B7-1 were identified. IgVH and B7-1 were confirmed by electrospray ionization tandem spectrometry (ESI-MS/MS) and immunocytochemistry.</p><p><b>CONCLUSION</b>There are IgVH and B7-1 expressions in human colorectal carcinoma cell lines LS174T and SW480. Results obtained will help to elucidate the mechanisms of tumor immune escape.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Mapeamento de Peptídeos
/
Região Variável de Imunoglobulina
/
Linhagem Celular Transformada
/
Neoplasias Colorretais
/
Cadeias Pesadas de Imunoglobulinas
/
Antígeno B7-1
/
Proteoma
/
Alergia e Imunologia
/
Genética
/
Metabolismo
Tipo de estudo:
Estudo prognóstico
Limite:
Humanos
Idioma:
Chinês
Revista:
Chinese Journal of Oncology
Ano de publicação:
2005
Tipo de documento:
Artigo
Similares
MEDLINE
...
LILACS
LIS