Effects of Ac-SDKP on angiotensin II-induced collagen synthesis in vascular adventitial fibroblasts / 中国应用生理学杂志
Chinese Journal of Applied Physiology
;
(6): 179-192, 2013.
Artigo
em Chinês
| WPRIM
| ID: wpr-358645
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of an anti-fibrotic tetra peptide Ac-SDKP on vascular fibrosis by regulating extracellular regulated protein kinase (ERK1/2) activity through Ang II.</p><p><b>METHODS</b>Rat vascular adventitial fibroblasts were cultured in vitro. They were randomly divided into control group, Ang II (10(-6) mmol/L) group, Ang II and Ac-SDKP joint action group, PD98059 group. Type I, III collagen contents in adventitia fibroblasts were measured by RT-PCR and the expressions of matrix metalloproteinases (MMP-2) and transforming growth factor-beta1 (TGF-beta1) were determined by Western blot.</p><p><b>RESULTS</b>Ac-SDKP could reduced Ang II-induced expression of type I, III collagen secretion and TGF-beta1 at mRNA,and increase MMP-2 expression, PD98059 could inhibit the above effect.</p><p><b>CONCLUSION</b>The results suggested that Ac-SDKP could inhibit the formation and development of vascular fibrosis through blocking ERK1/2 pathway mediated by Ang II. Ac-SDKP therefore served as an antifibrotic factor in vascular fibrosis.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Oligopeptídeos
/
Farmacologia
/
Flavonoides
/
Angiotensina II
/
Células Cultivadas
/
Ratos Sprague-Dawley
/
Metaloproteinase 2 da Matriz
/
Sistema de Sinalização das MAP Quinases
/
Biologia Celular
/
Colágeno Tipo I
Limite:
Animais
Idioma:
Chinês
Revista:
Chinese Journal of Applied Physiology
Ano de publicação:
2013
Tipo de documento:
Artigo
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