The influence of rhAng-1 on endothelial junctions of BBB after focal cerebral ischemia/reperfusion in rats / 中国应用生理学杂志
Chinese Journal of Applied Physiology
; (6): 147-152, 2013.
Article
em Zh
| WPRIM
| ID: wpr-358654
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>This study was performed to determine whether recombinant human angiopoietin-1 (rhAng-1) decreases the permeability of the blood-brain barrier (BBB) in focal cerebral ischemia and reperfusion rats, whether RhAng-1 opens the BBB by affecting tight junction associated proteins zonnula occludin-1 (ZO-1), occludin and adherens junction protein vascular endothelial (VE)-cadherin.</p><p><b>METHODS</b>The rats were divided into eight groups randomly( n = 10): (1) sham-operated group; (2) ischemia group; (3)-(5) ischemia/reperfusion (middle cerebral artery occlusion and reperfusion (MCAO/R) 12 h, 48 h, and 7 days) and 0.9% saline groups; (6)-(8) ischemia/reperfusion (MCAO/R 12 h, 48 h, and 7 days) and rhAng-1 groups. The Bee permeability was assessed by Evans blue extravasation. The messenger RNA and protein expressions of ZO-1, occludin, and VE-cadherin were determined by reverse transcription-polymerase chain reaction, Western blot, and immunohistochemistry assays.</p><p><b>RESULTS</b>The BBB permeability and the brain infarct volume were significantly decreased after rhAng-1 injection. The expressions of ZO-1, occludin, and VE-cadherin were increased after rhAng-1 injection.</p><p><b>CONCLUSION</b>rhAng-1 may decrease the permeability of BBB in MCAO/R rats by upregulation of ZO-1, occludin, and VE-cadherin.</p>
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Índice:
WPRIM
Assunto principal:
Farmacologia
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Proteínas Recombinantes
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Endotélio Vascular
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Barreira Hematoencefálica
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Traumatismo por Reperfusão
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Antígenos CD
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Caderinas
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Isquemia Encefálica
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Ratos Wistar
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Biologia Celular
Limite:
Animals
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Humans
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Male
Idioma:
Zh
Revista:
Chinese Journal of Applied Physiology
Ano de publicação:
2013
Tipo de documento:
Article