The functional changes in L-type Ca2+ channel of hypertrophied cardiomyocytes in neonatal rats induced by angiotensin II / 中国应用生理学杂志
Chinese Journal of Applied Physiology
;
(6): 91-95, 2013.
Artigo
em Chinês
| WPRIM
| ID: wpr-358670
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the molecular and functional changes in L-type Ca2+ channel of hypertrophied cardiomyocytes in neonatal rats induced by angiotensin II (Ang II).</p><p><b>METHODS</b>The in vitro model of cardiomyocyte hypertrophy was established in cultured cardiomyocytes from neonatal rats. Whole cell patch clamp was used to measure the L-type Ca2+ currents. Semi-quantitative RT-PCR was used to determine the mRNA expression of L-type Ca2+ channel alpha1C subunits.</p><p><b>RESULTS</b>In the hypertrophied cardiomyocytes induced by Ang II, I(Ca, L) densities were increased, whereas the features of I(Ca,L) activation, inactivation or recovery from inactivation were not affected. Meanwhile, Ang II increased the mRNA expression of L-type Ca2+ channel alpha1C subunits in cardiomyocytes. All these actions of Ang II could be blocked by the angiotensin II 1 type receptor blocker losartan.</p><p><b>CONCLUSION</b>During cardiomyocyte hypertrophy induced by Ang II, there are significant changes in the molecule and function of L-type Ca2+ channels, which are mediated by the angiotensin II 1 type receptor.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Patologia
/
Fisiologia
/
Angiotensina II
/
Cálcio
/
Ratos Sprague-Dawley
/
Canais de Cálcio Tipo L
/
Miócitos Cardíacos
/
Hipertrofia
/
Potenciais da Membrana
/
Metabolismo
Tipo de estudo:
Estudo prognóstico
Limite:
Animais
Idioma:
Chinês
Revista:
Chinese Journal of Applied Physiology
Ano de publicação:
2013
Tipo de documento:
Artigo
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