The genetic screening of a dominant zebrafish mutant in long-term memory / 中国应用生理学杂志
Chinese Journal of Applied Physiology
;
(6): 72-76, 2013.
Artigo
em Chinês
| WPRIM
| ID: wpr-358675
ABSTRACT
<p><b>OBJECTIVE</b>To screen the learning and memory mutant from N-ethyl-N-nitrosourea (ENU) mutagenic zebrafish F1, and to get the new model animal to study the mechanism of learning and memory.</p><p><b>METHODS</b>Zebrafish mutant was screened by inhibitory avoidance behavioral test and identified by the expression of gene c-fos with qRT-PCR.</p><p><b>RESULTS</b>We isolated a zebrafish mutant related to learning and memory, fgt. In this fgt zebrafish mutant long-term memory was much lower than that in wild-type when tested at 24 h after training. The 24 h long-term memory in about half of fgt mutant F2 (13/30) were significantly lower than those in wild-type, and the others relatively normal. Compared with the expression in wild-type fishes, the expression of immediate-early genes (IEGs) c-fos in half of fgt mutant F2 (13/30) after exploring in a novel environment increased distinctly from the basal control levels statistically, and the others relatively normal, which were in accordance with the behavioral results.</p><p><b>CONCLUSION</b>The zebrafish mutant fgt is a dominant mutant with defect in long-term memory.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Peixe-Zebra
/
Testes Genéticos
/
Genes Precoces
/
Modelos Animais de Doenças
/
Memória de Longo Prazo
/
Genética
Tipo de estudo:
Estudo diagnóstico
/
Estudo prognóstico
/
Estudo de rastreamento
Limite:
Animais
Idioma:
Chinês
Revista:
Chinese Journal of Applied Physiology
Ano de publicação:
2013
Tipo de documento:
Artigo
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