The effect of niflumic acid on gamma aminobutyric acid activated current in DRG neurons / 中国应用生理学杂志
Chinese Journal of Applied Physiology
;
(6): 68-71, 2013.
Artigo
em Chinês
| WPRIM
| ID: wpr-358676
ABSTRACT
<p><b>OBJECTIVE</b>To explore the modulatory effect of niflumic acid (NFA) on gamma aminobutyric acid (GABA)-activated currents of dorsal root ganglion (DRG) neurons in rat.</p><p><b>METHODS</b>The whole-cell patch-clamp technique was used to record the NFA- and GABA-activated currents in neurons freshly dissociated from rat DRG neurons.</p><p><b>RESULTS</b>Application of NFA(0.1 - 100 micromol/L) could induce concentration-dependent outward currents in some cells (21/48,43.75%), and GABA (0.1 - 100 micromol/L) could induce concentration-dependent inward currents in some cells(150/159,94.32%). NFA-(100 micromol/L) and GABA-(100 micromol/L) activated currents were (0.27 +/- 0.06) nA (n = 12) and (1.29 +/- 0.72) nA (n = 53) respectively. However, pre-application of NFA (0.1 - 100 micromol/L) could inhibit the GABA-activated inward current which was identified to be GABAA receptor-mediated current. The inhibitory effects of NFA were concentration-dependent. NFA could not alter the EC50 (about 30 micromol/L) and inverse potential (about -10 mV) of GABA-activated current (P > 0.05).</p><p><b>CONCLUSION</b>Pre-application of NFA exerts a more strong inhibitory effect on the peak value of GABA-activated current.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Farmacologia
/
Fisiologia
/
Separação Celular
/
Células Cultivadas
/
Ácido Niflúmico
/
Ratos Sprague-Dawley
/
Técnicas de Patch-Clamp
/
Ácido gama-Aminobutírico
/
Gânglios Espinais
/
Metabolismo
Limite:
Animais
Idioma:
Chinês
Revista:
Chinese Journal of Applied Physiology
Ano de publicação:
2013
Tipo de documento:
Artigo
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