The protective effects of Shenfu injection on the global cerebral ischemia/reperfusion injury of rats / 中国应用生理学杂志
Chinese Journal of Applied Physiology
;
(6): 462-465, 2012.
Artigo
em Chinês
| WPRIM
| ID: wpr-358713
ABSTRACT
<p><b>OBJECTIVE</b>To study the the protective effects and mechanism of Shenfu injection on the global cerebral ischemia/reperfusion (I/R) injury of rats.</p><p><b>METHODS</b>Divided 40 SD male rats into 4 groups randomly (n = 10) sham operation group, model control group, nimodipine group( 30 mg/kg) and Shenfu injection group (10 mg/kg). Made global cerebral ischemia/reperfusion injury model (CI/R) by adopting Pulsinelli's Four Arterial Acclusion method,and then practised administration three times, respectively one day before the surgery, one hour before the surgery and 30 minutes before reperfusion. Finally, measured the contents of brain tissue glutamate (Glu), aspartic acid (Asp) and Glycine (Gly) by means of High Performance Liquid Chromatography, the content of Ca2+ by means of Atomic absorption spectrophotometry, the brain water content by means of wet and dry weight, the activity of brain tissue superoxide dismutase (SOD) and content of malondialdehyde (MDA) by means of chemical colorimetry.</p><p><b>RESULTS</b>Compared with the sham operation group, the contents of Glu, Ca2+, MDA and water in the brains of CI/R model group rats increased remarkably (P < 0.05 or P < 0.01), while the activity of SOD decreased apparently (P < 0.05); Shenfu injection could significantly decrease the contents of Glu, Ca2+ and water (P < 0.05, P < 0.01) in brain tissue and remarkably increased the activity if SOD and the ration of SOD/MDA (P < 0.05).</p><p><b>CONCLUSION</b>The mechanism of Shenfu injection preventing cerebral ischemia/reperfusion injury is relevant to excitatory amino acid toxicity reduction, Ca2+ overload blockage and antioxidant capacity improvement.</p>
Texto completo:
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Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Farmacologia
/
Medicamentos de Ervas Chinesas
/
Traumatismo por Reperfusão
/
Isquemia Encefálica
/
Ratos Sprague-Dawley
/
Usos Terapêuticos
/
Tratamento Farmacológico
/
Fitoterapia
/
Metabolismo
/
Antioxidantes
Limite:
Animais
Idioma:
Chinês
Revista:
Chinese Journal of Applied Physiology
Ano de publicação:
2012
Tipo de documento:
Artigo
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