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Effects of pulse electrical stimulation on mutual adhesion of vascular endothelial cell and endothelial progenitor cell / 生物医学工程学杂志
Journal of Biomedical Engineering ; (6): 689-697, 2011.
Artigo em Chinês | WPRIM | ID: wpr-359198
ABSTRACT
The present paper is aimed to investigate the effects of pulse electrical stimulation on mutual adhesion of vascular endothelial cell and endothelial progenitor cell (EPC). EPC was induced from periphery blood, labeled with fluorescence dye and then co-cultured with vascular endothelial cell. With a fixed electric voltage and frequency of 5V and 5Hz, respectively, the co-culture system was continually stimulated for 24h under different pulse width, 1, 3, 6 and 9ms. After pulse stimulation, fluorescence intensity of adherent labeled EPC was measured and converted to fluorescence ratio. Compared to that in the control group, fluorescence ratio of 3 ms and 6 ms group were significantly larger, while that in the 9 ms group was lower. The peak fluorescence ratio value was appeared at 6 ms group. It is indicated that suitable pulse electrical stimulation could benefit the adhesion of endothelial cell and EPC. All these results provide a new theoretical basis about why electrical stimulation could contribute to neovascularization.
Assuntos
Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Efeitos da Radiação / Células-Tronco Hematopoéticas / Adesão Celular / Células Cultivadas / Técnicas de Cocultura / Biologia Celular / Estimulação Elétrica / Células Endoteliais da Veia Umbilical Humana Limite: Adulto / Feminino / Humanos / Masculino Idioma: Chinês Revista: Journal of Biomedical Engineering Ano de publicação: 2011 Tipo de documento: Artigo

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Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Efeitos da Radiação / Células-Tronco Hematopoéticas / Adesão Celular / Células Cultivadas / Técnicas de Cocultura / Biologia Celular / Estimulação Elétrica / Células Endoteliais da Veia Umbilical Humana Limite: Adulto / Feminino / Humanos / Masculino Idioma: Chinês Revista: Journal of Biomedical Engineering Ano de publicação: 2011 Tipo de documento: Artigo