Radiosensitization effect of black garlic extract on lung cancer cell line Lewis cells / 中国中西医结合杂志
Chinese Journal of Integrated Traditional and Western Medicine
;
(12): 1093-1097, 2013.
Artigo
em Chinês
| WPRIM
| ID: wpr-359249
ABSTRACT
<p><b>OBJECTIVE</b>To explore the radiosensitization effect of black garlic extract (BGE) on lung cancer cell line Lewis cells.</p><p><b>METHODS</b>The inhibition rate of lung cancer cells after BGE action was detected by MTT. Effect of BGE combined radiotherapy on the colony formation rate was observed by cloning formation assay. Changes of the cell morphology were observed by Hoechst staining. Changes of the cell cycle were detected by flow cytometry. Real time PCR was used to detect mRNA expressions of bcl-2 and bax.</p><p><b>RESULTS</b>BGE could have significant inhibitory action on the growth of lung cancer Lewis cells. The combination of BGE and radiotherapy (by 60Co gamma) significantly induced Lewis cells' apoptosis in G2/M stage, obviously decreased the expression of bcl-2, and up-regulated the expression of bax.</p><p><b>CONCLUSIONS</b>BGE could sensitize the lung cancer Lewis cells to ionizing irradiation. This effect might be probably caused by changing the cell cycles and affecting expressions of bax and bcl-2.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Patologia
/
Farmacologia
/
Efeitos da Radiação
/
Radiossensibilizantes
/
Extratos Vegetais
/
Apoptose
/
Carcinoma Pulmonar de Lewis
/
Proteínas Proto-Oncogênicas c-bcl-2
/
Linhagem Celular Tumoral
/
Proteína X Associada a bcl-2
Limite:
Animais
Idioma:
Chinês
Revista:
Chinese Journal of Integrated Traditional and Western Medicine
Ano de publicação:
2013
Tipo de documento:
Artigo
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