Using oligonucleotide suspension arrays for laboratory identification of bacteria responsible for bacteremia / 浙江大学学报(英文版)(B辑:生物医学和生物技术)
Journal of Zhejiang University. Science. B
;
(12): 291-298, 2008.
Artigo
em Inglês
| WPRIM
| ID: wpr-359430
ABSTRACT
The aim of this study was to develop and validate an oligonucleotide suspension array for rapid identification of 15 bacterial species responsible for bacteremia, particularly prevalent in Chinese hospitals. The multiplexed array, based on the QIAGEN LiquiChip Workstation, included 15 oligonucleotide probes which were covalently bound to different bead sets. PCR amplicons of a variable region of the bacterial 23S rRNA genes were hybridized to the bead-bound probes. Thirty-eight strains belonging to 15 species were correctly identified on the basis of their corresponding species-specific hybridization profiles. The results show that the suspension array, in a single assay, can differentiate isolates over a wide range of strains and species, and suggest the potential utility of suspension array system to clinical laboratory diagnosis.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Oligonucleotídeos
/
Células-Tronco
/
RNA Ribossômico
/
RNA Ribossômico 23S
/
Sondas de DNA
/
Química
/
Técnicas Bacteriológicas
/
Técnicas Genéticas
/
Técnicas de Tipagem Bacteriana
/
Bacteriemia
Tipo de estudo:
Estudo diagnóstico
/
Estudo prognóstico
Idioma:
Inglês
Revista:
Journal of Zhejiang University. Science. B
Ano de publicação:
2008
Tipo de documento:
Artigo
Similares
MEDLINE
...
LILACS
LIS