Quantitative Analysis of Immuno-fluorescence of Nuclear Factor-κB Activation / 生物医学工程学杂志
Journal of Biomedical Engineering
;
(6): 669-674, 2015.
Artigo
em Chinês
| WPRIM
| ID: wpr-359588
ABSTRACT
Immuno-fluorescence technique can qualitatively determine certain nuclear translocation, of which NF-κB/ p65 implicates the activation of NF-κB signal pathways. Immuno-fluorescence analysis software with independent property rights is able to quantitatively analyze dynamic location of NF-κB/p65 by computing relative fluorescence units in nuclei and cytoplasm. We verified the quantitative analysis by Western Blot. When we applied the software to analysis of nuclear translocation in lipopolysaccharide (LPS) induced (0. 5 h, 1 h, 2 h, 4 h) primary human umbilical vein endothelial cells (HUVECs) , we found that nuclear translocation peak showed up at 2h as with calculated Western blot verification results, indicating that the inventive immuno-fluorescence analysis software can be applied to the quantitative analysis of immuno-fluorescence.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Software
/
Núcleo Celular
/
Imunofluorescência
/
Transporte Ativo do Núcleo Celular
/
Citoplasma
/
Subunidade p50 de NF-kappa B
/
Células Endoteliais da Veia Umbilical Humana
/
Metabolismo
Limite:
Humanos
Idioma:
Chinês
Revista:
Journal of Biomedical Engineering
Ano de publicação:
2015
Tipo de documento:
Artigo
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