Influence of iNOS silencing by RNA interference on proliferation activity of Tca8113 cell / 中华口腔医学杂志
Chinese Journal of Stomatology
;
(12): 547-549, 2007.
Artigo
em Chinês
| WPRIM
| ID: wpr-359699
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the apoptosis and proliferation activity of Tca8113 cells.</p><p><b>METHODS</b>The vector that involves short hairpin RNA of iNOS was transfected to Tca8113 cells. The change of iNOS expression was observed using immunohistochemistry technique, the apoptosis rate examined by flow cytometry, and the proliferation Tca8113 cells examined by methyl thiazolyl tetrazolium (MTT).</p><p><b>RESULTS</b>The expression of iNOS in Psilencer-iNOS group was lower than that in control groups (P < 0.01), the apoptosis rate was higher than that in control groups (P < 0.01); whereas the proliferation activity of Tca8113 cells in Psilencer-iNOS group was lower than that in control groups.</p><p><b>CONCLUSIONS</b>Down expression of iNOS by RNAi can promotes apoptosis of Tca8113 cells and has an anti-proliferation activity effect.</p>
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DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Patologia
/
Plasmídeos
/
Neoplasias da Língua
/
Carcinoma de Células Escamosas
/
Transfecção
/
Regulação Neoplásica da Expressão Gênica
/
Apoptose
/
Interferência de RNA
/
Linhagem Celular Tumoral
/
Proliferação de Células
Limite:
Humanos
Idioma:
Chinês
Revista:
Chinese Journal of Stomatology
Ano de publicação:
2007
Tipo de documento:
Artigo
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