Regulation of SIRT1 by SREBP-1 in Adipogenic Differentiation of Mesenchymal Stem Cells / 中国实验血液学杂志
Journal of Experimental Hematology
;
(6): 590-595, 2016.
Artigo
em Chinês
| WPRIM
| ID: wpr-360042
ABSTRACT
<p><b>OBJECTIVE</b>To study the regulation of SIRT1 by transcription factor SREBP-1 in adipogeneic differentiation of bone marrow mesenchymal stem cells (BMMSC).</p><p><b>METHODS</b>Oil red O staining was used to identify the adipogenic differentiation of BMMSC; the mRNA transcription levels of AP2, LPL, SREBF-1, SIRT1 gene were detected by RT-PCR; the expession level of SREBP-1 was determined by Western-blot. The chromatin immunoprecipitation (ChIP) assay was used to investigate the binding of SREBP-1 to SIRT1 promoter.</p><p><b>RESULTS</b>BMMSC exposed to adipogenesis inducing medium become mature adipocytes at day 14; the mRNA transcription levels of AP2, LPL, SREBF-1, SIRT1 genes were up-regulated in adipocyte differentiation of BMMSC; the protein level of SREBP-1 was higher obviously; SIRT1 gene sequences was succesfully amplified from the genomic DNA immunoprecipitated by SREBP-1 antibody.</p><p><b>CONCLUSION</b>SREBP-1 can bind to the promoter region of the SIRT1 gene in adipogenesis of BMMSC, and may be involved in the transcriptional regulation of the SIRT1 gene.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Diferenciação Celular
/
Regulação para Cima
/
Células Cultivadas
/
Regulação da Expressão Gênica
/
Regiões Promotoras Genéticas
/
Adipócitos
/
Biologia Celular
/
Imunoprecipitação da Cromatina
/
Proteína de Ligação a Elemento Regulador de Esterol 1
/
Adipogenia
Tipo de estudo:
Estudo prognóstico
Limite:
Humanos
Idioma:
Chinês
Revista:
Journal of Experimental Hematology
Ano de publicação:
2016
Tipo de documento:
Artigo
Similares
MEDLINE
...
LILACS
LIS