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Induction of bone matrix protein expression by native bone matrix proteins in C2C12 culture / 生物医学与环境科学(英文)
Biomedical and Environmental Sciences ; (12): 164-169, 2009.
Artigo em Inglês | WPRIM | ID: wpr-360681
ABSTRACT
<p><b>OBJECTIVE</b>To study the expression of bone matrix protein (BMP) induced by bovine bone morphogenetic proteins (BMPs) in vitro.</p><p><b>METHODS</b>Type I collagen, osteopontin (OPN), osteonectin (ON), osteocalcin (OC), and bone sialoprotein (BSP) were detected by immunohistochemistry in C2C12 cultured from day 1 to day 28.</p><p><b>RESULTS</b>The signaling of bone matrix protein expression became weaker except for type I collagen, OC and BSP after 5 days. Fourteen days after culture, the positive signaling of type I collagen, OPN, ON, OC, and BSP was gradually declined, and could be detected significantly as compared with that of the negative control on day 28. BMP assay showed that the alkaline phosphatase (ALP) activity was higher in C2C12 culture than in the control during the 14-day culture. Also, total protein and DNA significantly increased during the 14-day culture. High levels of ALP were seen in preosteoblasts and osteoblasts in vivo and in differentiating osteoblasts in vitro. ALP was well recognized as a marker reflecting osteoblastic activity.</p><p><b>CONCLUSION</b>Native bovine BMP induces conversion of myoblasts into osteoblasts, produces type I collagen, and plays significantly role in osteoinduction and bone matrix mineralization of C2C12 in vitro.</p>
Assuntos
Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Osteoblastos / Farmacologia / Fisiologia / Matriz Óssea / DNA / Linhagem Celular / Regulação da Expressão Gênica / Proteínas Morfogenéticas Ósseas / Fosfatase Alcalina / Metabolismo Limite: Animais Idioma: Inglês Revista: Biomedical and Environmental Sciences Ano de publicação: 2009 Tipo de documento: Artigo

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Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Osteoblastos / Farmacologia / Fisiologia / Matriz Óssea / DNA / Linhagem Celular / Regulação da Expressão Gênica / Proteínas Morfogenéticas Ósseas / Fosfatase Alcalina / Metabolismo Limite: Animais Idioma: Inglês Revista: Biomedical and Environmental Sciences Ano de publicação: 2009 Tipo de documento: Artigo