Thyroid Hormone Regulates the mRNA Expression of Small Heterodimer Partner through Liver Receptor Homolog-1
Endocrinology and Metabolism
;
: 584-592, 2015.
Artigo
em Inglês
| WPRIM
| ID: wpr-36346
ABSTRACT
BACKGROUND:
Expression of hepatic cholesterol 7alpha-hydroxylase (CYP7A1) is negatively regulated by orphan nuclear receptor small heterodimer partner (SHP). In this study, we aimed to find whether thyroid hormone regulates SHP expression by modulating the transcriptional activities of liver receptor homolog-1 (LRH-1).METHODS:
We injected thyroid hormone (triiodothyronine, T3) to C57BL/6J wild type. RNA was isolated from mouse liver and used for microarray analysis and quantitative real-time polymerase chain reaction (PCR). Human hepatoma cell and primary hepatocytes from mouse liver were used to confirm the effect of T3 in vitro. Promoter assay and electrophoretic mobility-shift assay (EMSA) were also performed using human hepatoma cell lineRESULTS:
Initial microarray results indicated that SHP expression is markedly decreased in livers of T3 treated mice. We confirmed that T3 repressed SHP expression in the liver of mice as well as in mouse primary hepatocytes and human hepatoma cells by real-time PCR analysis. LRH-1 increased the promoter activity of SHP; however, this increased activity was markedly decreased after thyroid hormone receptor beta/retinoid X receptor alpha/T3 administration. EMSA revealed that T3 inhibits specific LRH-1 DNA binding.CONCLUSION:
We found that thyroid hormone regulates the expression of SHP mRNA through interference with the transcription factor, LRH-1.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Glândula Tireoide
/
Hormônios Tireóideos
/
Fatores de Transcrição
/
Receptores dos Hormônios Tireóideos
/
DNA
/
RNA
/
RNA Mensageiro
/
Ácidos e Sais Biliares
/
Colesterol 7-alfa-Hidroxilase
/
Linhagem Celular
Limite:
Animais
/
Humanos
Idioma:
Inglês
Revista:
Endocrinology and Metabolism
Ano de publicação:
2015
Tipo de documento:
Artigo
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