Role of IL-5-activated eosinophils on collagen gel contraction by lung fibroblasts / 천식및알레르기
Journal of Asthma, Allergy and Clinical Immunology
;
: 223-230, 2001.
Artigo
em Coreano
| WPRIM
| ID: wpr-36662
ABSTRACT
BACKGROUND:
Subepithelial fibrosis plays a major role in the development of irreversible airway obstruction in asthma. Eosinophils are major effector cells in allergic inflammation, and it has been suggested that eosinophil-derived mediators such as transforming growth factor-beta (TGF-beta) play a role in the pathogenesis of airway remodeling.OBJECTIVE:
The aim of this study was to evaluate whether eosinophils activated by IL-5 plays a major role in the collagen gel contraction by lung fibroblasts.METHOD:
Various cell numbers of lung fibroblasts were cultured in collagen gels to determine the appropriate numbers of fibroblasts. Purified human peripheral blood eosinophils were activated by IL-5 for 3 days, and TGF-beta mRNA expression was evaluated using semiquantitative RT- PCR. The cultured supernatants with or without TGF-beta were added to the collagen gel media with lung fibroblasts, and collagen gel diameter was serially measured to evaluate collagen gel contraction.RESULTS:
The amount of collagen gel contraction was significantly associated with the number of fibroblasts (p 0.05). Moreover, expression of TGF-beta mRNA in eosinophils was the same before and after stimulus of IL-5.CONCLUSION:
Activated eosinophils by IL-5 may play a minor role in the collagen gel contraction by lung fibroblasts.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Asma
/
Fibrose
/
RNA Mensageiro
/
Contagem de Células
/
Reação em Cadeia da Polimerase
/
Fator de Crescimento Transformador beta
/
Colágeno
/
Interleucina-5
/
Obstrução das Vias Respiratórias
/
Eosinófilos
Limite:
Humanos
Idioma:
Coreano
Revista:
Journal of Asthma, Allergy and Clinical Immunology
Ano de publicação:
2001
Tipo de documento:
Artigo
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