Studies on Diagnostic Methods to Detect Drug Allergies to Kampo Medicines. (I): Preliminary Analysis of Antigens from Crude Drugs and Antigen-Specific Antibodies using Shosaiko-to Extracts / 日本東洋医学雑誌

ABSTRACT

Recently, there has been an increase in reports of allergic reactions to Kampo medicines. In order to elucidate the mechanisms of these so-called Kampo allergies, suitable methods are necessary to detect allergens in crude drugs and allergenspecific antibodies in sensitized individuals. In this study, methodology and sensitivity were studied using enzyme-linked immunosorbent assay and Western blot analysis. The antigens used were haptenized baicalin and high-molecular-weight components of Ginseng Radix, both derived from Shosaiko-to extract.<br>Attempts were also made to further identify antigens from Shosaiko-to and detect antigenspecific antibodies in immunized rabbits.