TNF-alpha upregulates PTEN via NF-kappaB signaling pathways in human leukemic cells
Experimental & Molecular Medicine
;
: 121-127, 2007.
Artigo
em Inglês
| WPRIM
| ID: wpr-37548
ABSTRACT
TNF-alpha plays a variety of biological functions such as apoptosis, inflammation and immunity. PTEN also has various cellular function including cell growth, proliferation, migration and differentiation. Thus, possible relationships between the two molecules are suggested. TNF-alpha has been known to downregulate PTEN via NF-kappaB pathway in the human colon cell line, HT-29. However, here we show the opposite finding that TNF-alpha upregulates PTEN via activation of NF-kappaB in human leukemic cells. TNF-alpha increased PTEN expression at HL-60 cells in a time- and dose-dependent manner, but the response was abolished by disruption of NF-kappaB with p65 anisense phosphorothioate oligonucleotide or pyrrolidine dithiocarbamate. We found that TNF-alpha activated the NF-kappaB pathways, evidenced by the translocation of p65 to the nucleus in TNF-alpha-treated cells. We conclude that TNF-alpha induces upregulation of PTEN expression through NF-kappaB activation in human leukemic cells.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Transdução de Sinais
/
Leucemia
/
Expressão Gênica
/
Regulação para Cima
/
NF-kappa B
/
Fator de Necrose Tumoral alfa
/
Linhagem Celular Tumoral
/
PTEN Fosfo-Hidrolase
Limite:
Humanos
Idioma:
Inglês
Revista:
Experimental & Molecular Medicine
Ano de publicação:
2007
Tipo de documento:
Artigo
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