Protein Kinase C-alpha Regulates Toll-like Receptor 4-Mediated Inducible Nitric Oxide Synthase Expression
Journal of the Korean Association of Oral and Maxillofacial Surgeons
;
: 28-35, 2008.
Artigo
em Inglês
| WPRIM
| ID: wpr-37718
ABSTRACT
PURPOSE:
The nitric oxide (NO) release by inducible nitric oxide synthase (iNOS) is the key events in macrophage response to lipopolysaccharide (LPS) which is suggested to be a crucial mediator for inflammatory and innate immune responses. NO is an important mediator involved in many host defense action and may also lead to a harmful host response to bacterial infection. However, given the importance of iNOS in a variety of pathophysiological conditions, control of its expression and signaling events in response to LPS has been the subject of considerable investigation. MATERIALS ANDMETHODS:
The Raw264.7 macrophage cell line was used to observe LPS-stimulated iNOS expression. The expression of iNOS is observed by Western blot analysis and real-time RT-PCR. Protein kinase C (PKC)-alpha overexpressing Raw264.7 cells are established to determine the involvement of PKC-alpha in LPS-mediated iNOS expression. NF-kappaB activity is measured by IkappaBalpha degradation and NF-kappaB luciferase activity assay.RESULTS:
We found that various PKC isozymes regulate LPS-induced iNOS expression at the transcriptional and translational levels. The involvement of PKC-alpha in LPS-mediated iNOS induction was further confirmed by increased iNOS expression in PKC-alpha overexpressing cells. NF-kappaB dependent transactivation by LPS was observed and PKC-alpha specific inhibitory peptide abolished this activation, indicating that NF-kappaB activation is dependent on PKC-alpha.CONCLUSION:
Our data suggests that PKC-alpha is involved in LPS-mediated iNOS expression and that its downstream target is NF-kappaB. Although PKC-alpha is a crucial mediator in the iNOS regulation, other PKC isozymes may contribute LPS-stimulated iNOS expression. This finding is needed to be elucidated in further study.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Proteínas Quinases
/
Infecções Bacterianas
/
Proteína Quinase C
/
Ativação Transcricional
/
Linhagem Celular
/
Western Blotting
/
NF-kappa B
/
Proteínas I-kappa B
/
Óxido Nítrico Sintase Tipo II
/
Proteína Quinase C-alfa
Idioma:
Inglês
Revista:
Journal of the Korean Association of Oral and Maxillofacial Surgeons
Ano de publicação:
2008
Tipo de documento:
Artigo
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