Endothelin-1, Endothelin-2 and Endothelin-3 Induced Expression of Monocyte Chemoattractant Protein-1 in Rat Mesangial Cells / 대한신장학회잡지
Korean Journal of Nephrology
; : 358-365, 2003.
Article
em Ko
| WPRIM
| ID: wpr-37966
Biblioteca responsável:
WPRO
ABSTRACT
BACKGROUND: Monocyte chemoattractant protein- 1 (MCP-1) is an important mediator for monocyte/ macrophage infiltration in various inflammatory renal diseases and is produced by renal cells. In the process of renal diseases, endothelin-1 (ET-1) is known to play an active role in cell growth, inflammation and fibrosis. The aim of this study was to investigate whether three isoforms of endothelin regulate MCP-1 expression in cultured mesangial cells. METHODS: Mesangial cells were incubated with or without various doses of ET-1, ET-2 or ET-3. To determine the monocyte chemotactic activity, chemotaxis assay was performed in modified Boyden chambers using freshly isolated human monocytes. MCP-1 mRNA expression in mesangial cells was measured by Northern blot analysis. RESULTS: ET-1, ET-2 and ET-3 stimulated monocyte chemotactic activity released from mesangial cells in a dose-dependent manner. ET-1, ET-2 and ET-3 also stimulated MCP-1 mRNA expression in a time-dependent manner, which was seen as early as 4 hours and was maintained up to 24 hours. CONCLUSION: These data suggest that ET-1, ET- 2 and ET-3 stimulate MCP-1 expression in mesangial cells and may contribute to the monocyte/ macrophage infiltration in inflammatory renal diseases.
Palavras-chave
Texto completo:
1
Índice:
WPRIM
Assunto principal:
Fibrose
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RNA Mensageiro
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Monócitos
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Quimiotaxia
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Northern Blotting
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Endotelinas
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Quimiocina CCL2
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Endotelina-1
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Endotelina-2
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Endotelina-3
Limite:
Animals
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Humans
Idioma:
Ko
Revista:
Korean Journal of Nephrology
Ano de publicação:
2003
Tipo de documento:
Article