Expression and biological significance of Runx2 in dedifferentiated chondrosarcoma cell line / 肿瘤研究与临床
Cancer Research and Clinic
; (6): 231-234, 2010.
Article
em Zh
| WPRIM
| ID: wpr-379838
Biblioteca responsável:
WPRO
ABSTRACT
Objective To detect the different expression of Runx2 in dedifferentiated chondrosarcoma and conventional chondrosarcoma, and to investigate the role of Runx2 in the occurrence and development of dedifferentiated chondrosarcoma. Methods Dedifferentiated chondrosarcoma cell line NDCS-1 and normal chondrosarcoma cell line SW1353 were cultured, then mRNA and total cellular protein were extracted.RT-PCR Western blotting, and immunocytochemistry were used to detect the expression of Runx2.Immunohistochemistry was used to test Runx2's expression in the dedifferentiated chondrosarcoma specim ens that confirmed by pathology. Results Runx2 was high expression in dedifferentiated chondrosarcoma cell line and high-grade component of dedifferentiated chondrosarcoma tissues. Conclusion The high expression of Runx2 in dedifferentiated chondrosarcoma is involved in the occurrence and development of dedifferentiated chondrosarcoma.
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Índice:
WPRIM
Idioma:
Zh
Revista:
Cancer Research and Clinic
Ano de publicação:
2010
Tipo de documento:
Article