Involvement of TRPA1 in the cinnamaldehyde-induced pulpal blood flow change in the feline dental pulp
Restorative Dentistry & Endodontics
; : 202-209, 2016.
Article
em En
| WPRIM
| ID: wpr-38031
Biblioteca responsável:
WPRO
ABSTRACT
OBJECTIVES: The purpose of this study was to investigate the involvement of TRPA1 in the cinnamaldehyde-induced pulpal blood flow (PBF) change in the feline dental pulp. MATERIALS AND METHODS: Mandibles of eight cats were immobilized and PBF was monitored with a laser Doppler flowmetry at the mandibular canine tooth. To evaluate the effect of cinnamaldehyde on PBF, cinnamaldehyde was injected into the pulp through the lingual artery at a constant rate for 60 seconds. As a control, a mixture of 70% ethanol and 30% dimethyl sulfoxide (DMSO, vehicle) was used. To evaluate the involvement of transient receptor potential ankyrin 1 (TRPA1) in PBF change, AP18, a specific TRPA1 antagonist, was applied into the pulp through the Class V dentinal cavity followed by cinnamaldehyde-administration 3 minutes later. The paired variables of experimental data were statistically analyzed using paired t-test. A p value of less than 0.05 was considered as statistically significant. RESULTS: Administration of cinnamaldehyde (0.5 mg/kg, intra-arterial [i.a.]) induced significant increases in PBF (p 0.05), administration of cinnamaldehyde (0.5 mg/kg, i.a.) following the application of AP18 (2.5 - 3.0 mM, i.c.) resulted in an attenuation of PBF increase from the control level (p < 0.05). As a result, a TRPA1 antagonist, AP18 effectively inhibited the vasodilative effect of cinnamaldehyde (p < 0.05). CONCLUSIONS: The result of the present study provided a functional evidence that TRPA1 is involved in the mechanism of cinnamaldehyde-induced vasodilation in the feline dental pulp.
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Índice:
WPRIM
Assunto principal:
Artérias
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Vasodilatação
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Dimetil Sulfóxido
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Fluxometria por Laser-Doppler
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Anquirinas
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Dente Canino
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Polpa Dentária
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Dentina
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Etanol
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Mandíbula
Limite:
Animals
Idioma:
En
Revista:
Restorative Dentistry & Endodontics
Ano de publicação:
2016
Tipo de documento:
Article