Detection of survivin in esophageal cancer patients' serum with colloidal gold immunochromatography / 中华检验医学杂志

ABSTRACT

Objective To investigate the feasibility of screening for esophageal cancer with survivin colloidal gold immunochromatographic test strip. Methods The serum samples from 158 esophageal cancer patients and 146 healthy individuals were tested with survivin colloidal gold immunoohromatographic test strips. Results 20 nm-diameter colloidal gold solution was prepared and survivin polyclonal antibodies with different concentrations were labeled to the gold particles. It showed that the optimal concentration for labeling was 12 μg/ml. The prepared survivin colloidal gold immunochromatographic strip teat showed positive rates of survivin in esophageal cancer group and control group were 51.9 % (82/158) and 15.1% (22/146) respectively (χ~2 = 45.7, P < 0.05). No statistical differences were observed in age groups (between 42-54 years 47.1%; between 56-68 years 40.9%; between 69-81years 63.9%, χ~2= 1.11, P > 0.05), gender groups (male 50.4%, female 58.1%, χ~2= 0.59, P > 0.05) , primary site groups (upper segment 56.3%, middle segment 46. 1%, lower segment 32. 1%, χ~2 = 2.64, P > 0.05), differentiation groups (well-differentiation 56.3%, moderate differentiation 43.2%, poor-differentiation 32.7%,χ2= 1.63, P >0.05)and lymph node metastasis groups (with metastasis43.3%, without metastasis43.4%, χ~2 = 0.00, P > 0.05). The sensitivity, specificity and accuracy of survivin colloidal gold immunochromatographic test strip was 51.9% (82/158), 84.9% (124/146) and 67.8% (206/304) respectively. Conclusions Survivin colloidal gold immunochromatographic test strip is fast, simple, easy-to-read. It could be used as a valuable tool for screening of high-risk patients with esophageal cancer.