Factors Affecting Production of C-reactive Protein in Vascular Smooth Muscle Cells / 대한신장학회지

ABSTRACT

PURPOSE:The stenosis of vascular access for hemodialysis is caused by neointimal hyperplasia with the proliferation of vascular smooth muscle cells (SMC) as a prominent feature. C-reactive protein (CRP) is known to be produced in vascular SMC and can promote SMC proliferation. However, it is unclear of which factors regulate CRP production in neointimal hyperplasia. In the present study, we evaluated the factors affecting production of CRF in aortic SMC. METHODS:Human aortic SMC were cultured in a American type culture collection (ATCC) medium containing 10% FBS. Platelet-derived growth factor (PDGF) (10, 100 ng/mL), interferon-gamma(INF-gamma (1, 10, 100 ng/mL), hydroxymethylglutaryl-coA reductase inhibitor (lovastatin) (10 ?M/L) were added. After 72 hours, the level of CRP in SMC was measured by Western blot analysis and cell proliferation was assessed by MTT dye reduction assay. We used RT-PCR to observe PDGF receptor expression in SMC. RESULTS:Both INF-gammaand PDGF were found to stimulate CRP production and SMC proliferation. In contrast, lovastatin inhibited PDGF or INF-gammainduced CRP production and SMC proliferation. The expression of PDGF receptor-alphain aortic SMC was increased after treatment of 100 ng/mL of IFN-gamma CONCLUSION:SMC proliferation and CRP production in SMC are stimulated by PDGF or INF-gammaand inhibited by statin.