Different expression of microRNA-155 and cysteine-rich 61 in human placentas from severe preeclamptic and normal pregnancies / 中华围产医学杂志
Chinese Journal of Perinatal Medicine
;
(12): 403-407, 2010.
Artigo
em Chinês
| WPRIM
| ID: wpr-383346
ABSTRACT
Objective To investigate the different expression of microRNA-155 (miR-155) and cysteine-rich 61 (CYR61) in human placentas between severe preeclamptic and normal pregnancies.Methods Placentas were obtained from severe preeclamptic and healthy control pregnant women (n=18 for each group) at 36~40 gestational weeks. The expressions of miR-155 and CYR61 mRNA were assessed by real-time quantitative reverse transcription-polymerase chain reaction, and the levels of CYR61 protein were tested by Western blot. Results Compared with the control group, the miR-155 expression was increased in placentas from severe preeclampsia groups ( 165. 7 ± 16. 4 vs 527.9±49.1,t=7.00, P<0.01), and the CYR61 mRNA expression (31.7±2.7 vs 16.4±1.2,t=5.10,P<0. 01), as well as the CYR61 protein expression (36.4±1.5 vs 19.7±1.2,t=36.26, P<0.01 ) were decreased. There was a significantly negative correlation between the expression of miR-155 and CYR61 mRNA within both groups (preeclamptic group r=-0.52, P<0.05;controlr=-0.57, P<0.05). Conclusions Up-regulation of placental miR-155 in severe preeclampsia may be related to the decreased expression of CYR61. Both miR-155 and CYR61 may contribute to the disorders of placental angiogenesis in severe preeclampsia in human.
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DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
Chinese Journal of Perinatal Medicine
Ano de publicação:
2010
Tipo de documento:
Artigo
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