Comparison of class switch recombination assays for immunoglobulin synthesis
Journal of Medical Research
;
: 5-9, 2005.
Artigo
em Vietnamita
| WPRIM
| ID: wpr-3834
ABSTRACT
The second step of immunoglobulin gene alteration consists of somatic hypermutation and class switch recombination. 80th are regulated by activation-induced cytidine deaminase (AID). Methods:
Study on possible application of class switch recombination assays for immunoglobulin gene alteration via AID. Cell based assays using AID B lymphocyte and NIH3T3 cell carrying switch substrate; gene transfer using retrovirus system; FACS analysis; PCR and ELISA.Results:
DNA sequencing for S region and gamma1CT are the most sensitive and accurate assays. However, gamma1CT assay seemed to be more reliable and applicable. Others are accurate assays but less applicable.Conclusion:
gamma1CT determination is the best class switch recombination assay for immunoglobulin gene alteration via AID.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Idioma:
Vietnamita
Revista:
Journal of Medical Research
Ano de publicação:
2005
Tipo de documento:
Artigo
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